Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISLESLE-ENFRFLTLEVANQARATRDILGKPEKELVERLIARDDYIDNL-KTIIENKCFSRIHAEEGAEQ-SESNRIRAIHIICVNLERIADHCVNIVRQIKHFSDPTFL-HRYEFHSMFNLIQ-SGLDMILPVQRSQKVAQALTICKIEHELDNQYKERFDQIQDEMRAQHNADVGDLITALFIFRYLERIGDALLNVGEAFIFAFLGEKIKIHQFQALQETLSQAGYEGSLS-EID-FESIWGGRSGCRIRRVKNTNPDPSKEVI-FKEGQKEKIQQERDNIDRWSDLFPGIAPHVVGYNEGGNYASMLVEFLPGCTLDEVVLTT-EPEQVRNALFVFEQTLVDVWEDTIDPQDHPSPGFIKQLSGRLDKIQQVHPGILTPHKRIGRITIPSLRKRLEQGTALEQAVQAPFTVLLHGDCNLNNVVYDFEEQRIRFIDLYRSRQADYIQDVSVFLVSNFRMPIFEPSL-RARLNW-TI-RHFYHFARGFAAKHGDTTFSFRLALGLCRSFLTSTRFELDTEFAYEMAERGGYLLDRLLEHGSKHQEEFTFPEAVLYY 4N9N Chain:A ((604-878)) --NLNLIDMKLFHHYCTKVWPTITAAKVSGPEIWRDYIPELAFDYPF---LMHALLAFSATHLSRTETGLEQYVSSHRLDALRLLREAVLEISEN----NTDALVASALILIMDSLANASVDNIFEMLRIDEGLRLKIYKDTEGYYTI-GIGHLLTKS--PSLNAAKSELDKAIGRNTNGVIT-------KDEAEKLFNQDVDAAVRGILRNA----KLKPVYDSLDAVRRAALINMVFQMGETGVAG---------------FTNSLRMLQQKRWDEAAVNLAK-SRWYNQTPNRAKRVITTFRTGTWDAYV--------------DSMSPSAWIFHVKGAATILTAVWPLS------ERSKFHNIISVDLSDLGDV------INPDVGTIT---------ELVCFDESIADLYPVGLDSPYLITLAYLDKLHRE--------KNQGDFILRVFTFPA--LLDKTFLALLMTGDLGAMRIMRSYYKLLRGFATEVKDKVW-----------FLEGVTQVLPQ-------------------------------------

General information: TITO was launched using: RESULT: Template: 4N9N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1852 51317 27.71 123.36 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 27.71 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_4N9N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4n9n-query.scw PDB file : Tito_Scwrl_4N9N.pdb: