Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MSDTSIGNTSVSPDS-GLAARLAELIAQHLPNARDVSVQGIAPMFGGNARKAWSL-----ELVFQLDGLPQRRPCVMLIQGADRQIETDVAQEFRVLHGLNGKGTRAPAAVAMDARGEIVGAPTIVLERLPGKASAVDFLNSPDPAAARRLTEDLASVVADLHAVDWTPAAFDSALEGLSPREVALRQ--ITHWHDTFLAQRLEPLPVMS-GLFGWLYRHL-----PEPQRICLVHGDLRPGNFLYEGD--RVSGLLDWEMAHLGDPVEDLAWIYRPLWSPERFVPLREFVQRYAALARREI----AW------SDVLYYRVFNELKFAVISLTAARAFI---TSSTPNMRHADRAAT-----------VPPCLNRCLAWAEQHRKETSRV--- 3J9Q Chain:A ((2-386)) SFFHGVTVTNVDIGARTIALPASSVIGLCDVFTPGAQASAKPNVPVLLTSKKDAAAAFGIGSSIYLACEAIYNRAQAVIVAVGVETA-ETPEAQASAVIGGISAAGERTGLQALLDGKSRFNAQPRLLV--APGHSAQQAVATAMDGLAEKL------RAIAILDGPNSTDEAAVAYAKNFGSKRLFMVDPGVQVWDSATNAARNAPASAYAAGLFAWTDAEYGFWSSPSNKEIKGVTGTSRPVEFLDGDETCRANLLNNANIATIIRDDGYRLWGNRTLSSDSKWAFVTRV--RTMDLVMDAILAGHKWAVDRGITKTYVKDVTEGLRAFMRDLKNQGAVINFEVYADPDLNSASQLAQGKVYWNIRFTDVPPAENPNFRVEVTDQWLTEVLDVA

General information: TITO was launched using: RESULT: Template: 3J9Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1834 39435 21.50 115.98 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 21.50 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.211

(partial model without unconserved sides chains): PDB file : Tito_3J9Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3j9q-query.scw PDB file : Tito_Scwrl_3J9Q.pdb: