Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTRRVELARLRRMAVTALGRYPLAEGRLIFISHGENTTFRHIGADGTSHLVRVHRPSRHGRGVDSAAAVDSELAWLRALREDTDLLVPEPLAARDGASAVAVTEAGETRLCSVLRWMDGR-IHEASPRPVHLHRLGAAMASLHDQADAWTPPDGFTRIHWDHEAFFGNVMVYGRTPAAECWT-LLPPELRARFREVGARLADLIPGARDSGLIHADLHLGNALFRRG-AVKLIDFDDCGTGPRSYELAVALWELRGRPDYPAFRQALLSGYRARRDIDAT-HLDDFIALRQVAFDLWYTGMAQVNPAFSVRLDGVHQWSLDVLDLVEAG 4R7B Chain:A ((40-278)) --------------------------LSVEQLGGMTNQNYLAKTT-NKQYIVKFFGKGTEK-----LINRQDEKYNLELLKDL-GLDVKNYLFDI-------------EAGIKVNEYIESAITLDSTSIKTKFDKIAPILQTIHTSAKELRGEFA-PFEEI--KKYESL---IEE-----QIPYANYESVRNAVFSLEKRL---ADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEF---TSQEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYK----------------------------

General information: TITO was launched using: RESULT: Template: 4R7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1059 -36363 -34.34 -154.73 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -34.34 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.213

(partial model without unconserved sides chains): PDB file : Tito_4R7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r7b-query.scw PDB file : Tito_Scwrl_4R7B.pdb: