Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEVEDVRLGAELERLTEGATWCPVWHLQRPLSPTTATAAPPLP-SSTYLKMAPA---GTLAADVAVMQLLKSCDVPAPHVLHYAQGGEGQADFLLISALAGVHGATAEAL--SRPEALVRSFAGGLQSLHGQHSTALLRSAEDLGAKFDRRVAVRVARVERRLRVG---EVPGHAERLEAILAHVAKPPPNVESDLVFTHGDYCLPNVIFQILNNQAGETEAELRVVGFVDLGSAGVADRYVDLASAVWSLRFNGLEKHVPAFLQCYTARSELSGHKITINQDKLDWYINMFTLT 4GKH Chain:A ((19-272)) ----------NLDADLYGYRWARDNVGQSGATIYRLYGK--PNAPELFLKHGKGSVANDVTDEMVRLNWLTA-FMPLPTIKHFIR--TPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHS-IPVCNCPF----NSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLP-FSPDSVVTHGDFSLDNLIFD--E---------GKLIGCIDVGRVGIADRYQDLAILWNCLGE-FSPSLQKRLFQKYGI---D-----NPDMNKLQFHLMLDEFF

General information: TITO was launched using: RESULT: Template: 4GKH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1082 -28669 -26.50 -117.01 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -26.50 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_4GKH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4gkh-query.scw PDB file : Tito_Scwrl_4GKH.pdb: