Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKIYEKYRDLITNPFDIKYKNIEIKEIISYPPAGNDVVECLCKIGKDARNVFIKIERSKV--CDFATEIKNLNYLKNNSYYSKIPNIYESGIY-N----NKQYIVLSKIEGERLSDILIKNDNLRKELLYKYGQELAIIHRIPISNQKIA-----KQRAINS---YPNKTMYTTLSKE--NNIKEYIKFLEDNDFKKELTTFIHGDFHYANVLWLN-NDINGVIDWEYSGIGLREQDIAWACTLRPGQKFMDTMEDIQTFLKGYSEKEKFDIEKLRWCLINSYCHFYLMNENNEDYKNKLLILLRNCMEEKI 5IGI Chain:A ((10-257)) -----SQLYALAAR-HGLKLH-GPLTVN-----ELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNR-LPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVV--PQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDR-VRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLM-----VF--GEEGLAKLLLTYEAAGGR------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 953 -16585 -17.40 -72.42 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -17.40 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.308

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: