Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKSSSKIGGEYGEVRATIDVNKLNAYLKDKVPRV-KAPVDVK--QFKFGQSNPTYFLTDAS-------NTRWVLRKKPAGQLL-STTAHQIEREYTILNAIHRHNVKSSTPSEQNVPVPEPIILCEDKEIIGTPFYIMEFLDGRIFTNPRM--------PEIPENDRRECWLSAVRALAALSSLVPQELGLSNFAPMTPYFPRQIKSLSRVSQVQSEAADVETGIPTGKIPKFDEMIAWYRSHLPDE-SKTGSRIVHGDYKIDNMIFHPTENRVIGILDWELCTLGSPLADLANLTQPWSVDPSQIPEEYRVSLLRGFKNQTDDIPI--DLETLEREYCRLTHQPYPITEMVFARSWMLFRLSVISQGIAARYARRQASSEQAFLHVKLFPIVGELAIRVLEDAGHVLELKSKL 3ATT Chain:A ((5-329)) ----------------------VISRWLSSVLPGGAAPEVTVESGVDSTGMSSETIILTARWQQDGRSIQQKLVARVAPAAEDVPVFPTYRLDHQFEVIRLVGEL---------TDVPVPRVRWIETTGDVLGTPFFLMDYVEGVVPPDVMPYTFGDNWFADAPAERQRQLQDATVAALATLHSIPNAQNTFSFLT--DTTLHRHFNWVRSWYDFAV--------EGIGRSPLLERTFEWLQSHWPDDAAAREPVLLWGDARVGNVLYRDF--QPVAVLDWEMVALGPRELDVAWMIFAHRVFQELAGL----------A-TLPGLPEVMREDDVRATYQALTGVEL--GDLHWFYVYSGVMWACVFMRTGARRVH---------------------------------------

General information: TITO was launched using: RESULT: Template: 3ATT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1373 -15282 -11.13 -51.28 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -11.13 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_3ATT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3att-query.scw PDB file : Tito_Scwrl_3ATT.pdb: