Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDQLRTPLTLRALLTERQYAGFTAAWAELRGGSLS-GESLPETSPGPVLGDSLIGPDALRVRDLSWP------ERESTVLHLSSPGVSWILKTAGNT--AYSVGREARARAWT-GPLEARGTVARLLWYDDAAPLTILDYLPGCD----------VLGSPWEFSPRVYHQAG--AALALLHRAGARQDHGFTKGALTRTRARITEIRTLAPGGALPAAVDEKLAEAEDFLASYR------PGSVVVHPTHGDWQARNWLWDEPAGQLRIIDFGRFGWRPAAADFLELHDLWHTHPELEEAAARGYREAGGDWPAGSEVGSGERGNGGAGDTERLLRVARAVGHMWHRTTTARRIASAPAAR 5EQJ Chain:A ((4-461)) HHHSQDPMNALTTIDFNQHVIVRLPSKNYKIVELKPNTSVSLGKFGAFEVNDIIGYPFGLTFEIYYDIGKVRLLKYFTVEYLSSSNLLQFLIDKGDIQRVLDMSQESMGMLLNLANIQSEGNYLCMDETGGLLVYFLLERMFGGDNESKSKGKVIVIHENEHANLDLLKFANYSEKFIKEHVHTISLLDFFEPPTLQEIQSRFTPLPRALKGGKKNSYYRKLRWYNTQWQILELTGEFLYDGLVMATTLHLPTLVPK-LAEKIHGSRPIVCYGQFKETLLELAHTLYSDLRFLAPSILETRCRPYQSIRGKL----HPLMTMKGGGG--------------YLMWCHRVIPA---------

General information: TITO was launched using: RESULT: Template: 5EQJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1369 -22315 -16.30 -73.16 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -16.30 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.253

(partial model without unconserved sides chains): PDB file : Tito_5EQJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5eqj-query.scw PDB file : Tito_Scwrl_5EQJ.pdb: