Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISLESLEENFRFLTLEVANQARATRDILGKPEKELVERLIAR--------DDYIDNLKTIIE--NKCFSRIHAEEGAEQSESNRIRAIHIICVNLE------------------------RIADHCVNIVRQIKHFSDPTFLHRYEFHSMF----------NLIQSGLDMILPVQRSQK--------VAQALTICKIEHELDNQYKERFDQIQDEMRAQHNADV---GDLITALFIFRYLERI-GDALLNVGEAFIFAF--------------------LGEKIKIHQFQALQETLSQAGYEGSLSEIDFESIWGGRSG---------------------CRIRRVKNTNPDP----SKEVIFKEGQKEKIQQERDNIDRWS-------DLFPGIAPHVVGYNE--GGNYASMLVEFLPGCTLDEVVLTTEPEQVR---------NALFVFEQTLVDVWEDTIDPQDHPSPGFIKQLSGRLDKIQQVH-PGILTPHKRIGRITIPSLRKRLEQGTALEQAVQA-PFTVLLHGDCNLNNVVYDFEEQRIRFIDLYRSRQADYIQDVSVFLVSNFRMPIFEPSLRARLNWTIRHFYHFARGFAAKHGDTTFSFRLALGLCRSF-LTSTRFELDTEFAYEMAERGGYLLDRLLEHGSKHQEEFTFPEAVLYY 3VSM Chain:A ((27-659)) --NIQELQNFERWFKNNLSYSFSQKAEKVVNPNRNWNDNTVFDNLSPWTSVPDFGTVCHTLIGYCVRYNNTSDTLYQNPELAYNLINGLRIICSKLPDPPPHQQAPWGPVADWYHFTITMPEVFMNITIVLNETQHYDEAASLTRYWLGLYLPTAVNSMGWHRTAGNSMRMGVPYTYSQMLRGYSLAQIRQEQGIQEILNTIAFPYVTQGNGLHVDSIYIDHIDVRAYGYLINSYFTFAYYTYYFGDEVIN-TVGLTRAIENVGSPEGVVVPGVMSRNGTLYSNV-IGNFITYPLAVHSADYSKVLTKLS-KTYYGSVVGVTNRLAYYESDPTNNIQAPLWTMARRIWNRRGRIINYNANTVSFESG---IILQSLNGIMRIPSGTTSTQSFRPTIGQTAIAKTDTAGAILVYAKFAEMNNLQFKSCTLFYDHGMFQLYYNIGVEPNSLNNTNGRVIVLSRDT--SVNTNDLSFEAQRINNNNSSEGTTFNGVVCHRVPITNINVPSLTVR-SPNSSVELVEQIISFQTMYTAT---ASACYKLNVEGHS--DSLRAFRVNSDENIYVNVGNGVKALFNYPWVMVKEN--------NKVSFMSANEDTTIPFSVIMNSFTSIGEPALQYSPSNCFVYG----NGFKLNN-----STFDLQFIFEIV----

General information: TITO was launched using: RESULT: Template: 3VSM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2813 14243 5.06 27.87 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 5.06 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.133

(partial model without unconserved sides chains): PDB file : Tito_3VSM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3vsm-query.scw PDB file : Tito_Scwrl_3VSM.pdb: