Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEVEDVRLGAELERLTEGATWCPVWHLQRPLSPTTATAAPPLPSSTYLKMAPA------GTLAADVAVMQLLKSCDVPAPHVLHYAQGGEGQADFLLISALAGVHGATAEAL-SRPEALVRSFAGGLQSLHGQHSTALLRSAEDLGAKFDRRVAVRVARVERRLRVG-EV-PGHAERLEAILAHVAKPPPNVESDLVFTHGDYCLPNVIFQILNNQAGETEAELRVVGFVDLGSAGVADRYVDLASAVWSLRFNGL-EKHVPAFLQCYTARSELSGHKITINQDKLDWYINMFTLT 1L8T Chain:A ((3-263)) -----MRISPELKKLIEKYRCVKDTEGMSPAKVYKLVG---ENENLYLKMTDSRYKGTTYDVEREKDMMLWLEG-KLPVPKVLHFER--HDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHS-IDISDCPYT-NSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEK--PEEELVFSHGDLGDSNIFVK--D---------GKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGI---------KPDWEKIKYYILLDELF

General information: TITO was launched using: RESULT: Template: 1L8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1149 -31993 -27.84 -127.46 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -27.84 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_1L8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1l8t-query.scw PDB file : Tito_Scwrl_1L8T.pdb: