Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDITSDLKELNISYDKINVVRQGENSDTFLITTSK---------KKLIAKKFKNLNFKTITNTYLNKKILNQLVDKKLFPKVVYQSEEKDFLIYEYVESIKSNKDK----LFYKNLGMKIREIHEIKPCKN---------NLTFEQQLNFYK-MS---LGK--VN----KEKVYTRIEDLLNDINIKKSKLVFSHNDLNANNILFNK--------DICIIDYEYSSLNNKFCDLSKVIFEFQM------------------TEEEIESLLKGYRINFD---------------DVTKKKIILWQRFNLYLDYIWSIIISSNENQLTKENIESFYLNKYIDFDLKN 5FTG Chain:A ((30-353)) -------------DEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-------EAMVLESVMFAILAERSLGPKLYGIFP--QGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLE---STPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISS----------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 763 -3615 -4.74 -16.00 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -4.74 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.328

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: