Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKLLETTLLKQLKETHDDFSI-QNINSLHGGYLNHVFQINSN--LKPTVLWLNEN--KDSPKLSRQLIAIAILKKYFYSLIPDVYTSNR-----------SSIGCYYIMSKLNGENLERTLPYLTTEEQIHFFKSMGHLLGNIHLIGSKEKVGYLDNLQRIPW--KDWLGKYINSLIQDLLSQ-ESHHN-NSKWLTVQNALINHLEFI-DEPNNFSFLHGDYYPGNILYTED---FISGLLDFEWSLYGDPLYDFRVMEVFIFR---EFPYHKEFYNSYQQVRKLPSDFYKTILFYAKVYQLELISMAYSCFSKDHYFIKNMEPDLINWASKNK 6EF6 Chain:A ((30-323)) ---------IAQQALTHYDVSDNASLRLL-NLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAA-LRTD-SDVTVPTVVPARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPDEES--L----TLDDFQTLGRITASLHEHSQR--WTRPAGFGRFSWDWEHCLG-DTPRW-GRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAWMGSHTH---------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1194 -9602 -8.04 -36.37 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -8.04 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: