Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLTDQSTRIRAGEELDAAVIDPYLKAHIPGLEGTP-KISQFPGGASNLTYLIEYPRQEFVLRRPPFGHKAKSAHDMGREYRILNQLN--AGFPYCPKAYLYCTDES--------VIGAEFYVMERVKGIILRAELPPELNLDEQQTRSLCKSFIDKFVELHNVDYAACGLADLGR----PDGYVQRQIAGWTDRYEKA--LTPDAPLWEPVKAWLKDKQ----PADHHKPGIVHNDYRFDNVILDPENPMQIIGVLDWELATLGDPLMDLGNTLAYWVEAGDPAPVQLTRRQPSHLPGMLTRREFADYYAERAGIPPIDNLDFYYTYGLFRLAGIVQQIYYRYYHGQTQDKRFAQFVQMNKLLEQMSLQAIERSRL 6EF6 Chain:A ((27-318)) -----------------DVTIAQQALTHYDV--SDNASLRLL-NLSENATYLVEDGEHQSILRVHRQDY--HQPHEIESELDWLAALRTDSDVT-VPTVVPARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPDEES-----LT----LDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCL-GDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPD-RYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPR---------------LGEWQESWVAGYRSRRELPA-ADEAMLPSFVFLRRLLLLAWM-------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1215 -9377 -7.72 -34.99 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -7.72 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: