Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEPPLNRTNIFFGESHSDWLPVRGGE-SGDFVFRRGDGHAFAKIAPAS---RRGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLS--GADLLKAWPSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVN---PDFLPDEDKSTPQLDLLARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG 3TM0 Chain:A ((19-262)) -------------------VKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEG-KLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEED-----TPFKDPRELYDFLKTEK--PEEELVFSHGDLGDSNIFVKDGK--VSGFIDLGRSGRADKWYDIAFCVRSIREDIG----EEQYVELFFDLLGIK-PDWEKIKYYILLDEL---

General information: TITO was launched using: RESULT: Template: 3TM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1054 23154 21.97 98.53 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 21.97 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_3TM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tm0-query.scw PDB file : Tito_Scwrl_3TM0.pdb: