Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence GSAWFPGDPTYDCGISIFRPPKPGSRSRNGALAEVIYVNRGET----FKIIEGRYTCSIEQNCERPD-CNLPR-TWNYRGQ 3DNO Chain:E ((259-339)) -------------------------LLLNGSLAEEEVVIRSVNFTDNAKTIIVQLNTSVEINCTGAGHCNISRAKWN----

General information: TITO was launched using: RESULT: Template: 3DNO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 90 2515 27.94 54.66 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain E : 0.63 3D Compatibility (PKB) : 27.94 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.248

(partial model without unconserved sides chains): PDB file : Tito_3DNO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3dno-query.scw PDB file : Tito_Scwrl_3DNO.pdb: