Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGAAHSASEEVRELEGKTGFSSDQIEQLHRRFKQLSGDQP-TIRKENFNNVPDLELNPIRSKIVRAFFDNRNLRKGPSGLADEINFEDFLTIMSYFRPIDTTMDEEQVELSRKEKLRFLFHMYDSDSDGRITLEEYRNVVEELLSGNPHIEKESARSIADGAMMEAASVCMGQMEPDQVYEGITFEDFLKIWQGIDIETKMHVRFLNMETMALCH 4F0Z Chain:B ((7-161)) -----------YPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGN---------GEVDFKEFIEGVSQF----------SVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNN--LKDTQLQQIVDKTIINADKDGDGR---------ISFEEFCAVVGGLD-------------------

General information: TITO was launched using: RESULT: Template: 4F0Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 494 3539 7.16 22.98 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : 7.16 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_4F0Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4f0z-query.scw PDB file : Tito_Scwrl_4F0Z.pdb: