Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAAMVPGRSESWERGEPGRPALYFCGSIRGGREDRTLYERIVSRLRRFGTVLTEHVAAAELGARGEEAAGGDRLIHEQDLEWLQQADVVVAEVTQPSLGVGYELGRAVAFNKRILCLFRPQSGRVLSAMIRGAADGSRFQVWDYEEGEVEALLDRYFEADPPGQVAASPDPTT 4P5D Chain:C ((3-142)) --------------------RSVYFCGSIRGGREDQALYARIVSRLRRYGKVLTEHVADAELEPLGEEAAGGDQFIHEQNLNWLQQADVVVAEVTQPSLGVGYELGRAVALGKPILCLFRPQSGRVLSAMIRGAADGSRFQVWDYAEGEVETMLDRYFEA--------------

General information: TITO was launched using: RESULT: Template: 4P5D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 630 -95087 -150.93 -679.19 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain C : 0.89 3D Compatibility (PKB) : -150.93 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_4P5D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4p5d-query.scw PDB file : Tito_Scwrl_4P5D.pdb: