Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSINFSNLSKNINFINFSKISRGNNKELKYIVKDSEMNRYLFKINSIIHLDNKVNEFRFAKRIEHLPFEKSEIVDLCVFNQNRYICSIYKWIDG-KILEDQIKNYPSNKQYSFGFKAGRSLKLIHDLPIRDEELKQVEELPVRKIIEYYHQNKRF----ENDNIIIDFILKNNSKIKDEKI----------CFLHGHYSIKNFIIKNNDEISLIDYNQCYFGDYVQDF-SKNEIYNSYYSEQFAQGIIDGYFYNRKIPDTFWLKYAYYAAYNCITLFLWASKQEVISTKIK-IKEMIQNVLNQFNNFESIIPNWYKKNVLSKERQKFINSPYTLEKILGTPKKENKNDSKIKYFDEMYDDNDDDLLDEKMMDSFKEFLKNVAKK 3UZR Chain:A ((97-318)) -----------------------------------------------------------------------------VYQSDRF--NIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEI-DCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILSNAVLFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLK--YYQHKAPEVAERKAELNDVYWSIDQIIYGYERKDREMLIKDVSELLQTQAEMF--------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3UZR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 732 26370 36.02 128.63 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 36.02 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_3UZR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3uzr-query.scw PDB file : Tito_Scwrl_3UZR.pdb: