Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTRRVELARLRRMAVTALGRYPLAEGRLIFISHGENTTFRHIGADGTSHLVRVHRPSRHGRGVDSAAAVDSELAWLRALREDT-DLLVPEPLAARDGASAVAVTEAGETRLCSVLRWMDGRIHEASPRPVHLHRLG-AAMASLHDQADAWTPPDGFT--------RIHWDHEAFF-GNVMV-YGRTPAAECWTLLPPELRARFREVGARLADLI---------PGARDSGLIHADLHLGNALFRR-GAVKLIDFDDCGTGPRSYELAV-ALWELRGRPDYPAFRQAL-LSGYRARRDIDATHLDD-F------IALRQVAFDLWYTGMAQVNPAFSVRLDGVHQWSLDVLDLVEAG 2Q83 Chain:A ((11-346)) --LSAEDAKKLTELAENVLQGWDV---QAEKIDVIQALVWKVHTDSGAVCLKRIHRP---------EKKALFSIFAQDYLAKKGMN--VPGILPNKKGSLYSKH----GSFLFVVYDWIEGR--PFELTVKQDLEFIMKGLADFHTASVGYQPPNGVPIFTKLGRWPNHYTKRCKQMETWKLMAEAEKEDPFSQLYLQEIDGFIEDGLRIKDRLLQSTYVPWTEQLKKSPNLCHQDYGTGNTLLGENEQIWVIDLDTVSFDLPIRDLRKMIIPLLDTTGVWDDETFNVMLNAYESRAPLTEEQKQVMFIDMLFPYELYDVIREKYVRKSALPKEELESAFEYERIKANALRQLI---

General information: TITO was launched using: RESULT: Template: 2Q83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1257 -4174 -3.32 -13.87 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -3.32 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.345

(partial model without unconserved sides chains): PDB file : Tito_2Q83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2q83-query.scw PDB file : Tito_Scwrl_2Q83.pdb: