Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVAAPIPVAGWGDKDDPWECLGERSSGATVYRVGE-V--PSFYVKTTPPRHPDDHRFNPTKEAERLRWLAAQGLPVPEVVALDANDELAWVVTRALPGRPAARHWKP--EERWRVIDVVADVARTLHALPVAECPFERRLADLIHQASSSMALGALDLDDVDPSHEGWTAQQLWDELSKMTPPAEDDLVVCHGDFCLDNVLVDPETLTLAGILDVDRAGVSDRWMDLALALYNIGQDDVMGLRSAARRALPPAVRHH-RRPAQADLHPTAGRISLS 4GKI Chain:C ((25-268)) -------------YGYRWARDNVGQSGATIYRLYGKPNAPELFLKHGKGS----VANDVTDEMVRLNWLTA-FMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHK-LLPFSPDSVVTHGDFSLDNLIFDEG--KLIGCIDVGRVGIADRYQDLAILWNCLGEF-----SPSLQKRLFQKYGIDNPDMNKL------QFHLML

General information: TITO was launched using: RESULT: Template: 4GKI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1009 -947 -0.94 -3.98 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.71 3D Compatibility (PKB) : -0.94 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.376

(partial model without unconserved sides chains): PDB file : Tito_4GKI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4gki-query.scw PDB file : Tito_Scwrl_4GKI.pdb: