Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGRALLNDTWAVVTTPRPIVDAVVRSVCGRAVERLVPLAGGGLNETYRAEFAGDVAVVVRIARRPTPWFIDEAHLMAQAAAAGVPTAQVLGLEHLDHDGEPLSFSIQQFLPGRSLEELS--GELPAFALERVVAEAGEILARVHSVVPDDQRGIQHELRLPDEHEVARVARIV-------------ATALGPAAAAV-----VERGANFLRQEVTTRTAPPISLVHGDFCPKNLLIHDGTVAGFIDWEFAGPASPAYDFAQWEVDAGEPLHDRVDLVRRGYARVADPGTADAGWTPAFAVDWSLEKLGWTNPAAPAQFRRCVDVIARYTVC---------------------------------------------------------------- 2OLC Chain:A ((5-396)) --------KTPLYETLNESSAVALAVKLGLT--LTCQEIGDGNLNYVFHIY---DRALIIKQAVPYWPLTIDRARIESSALIRQGEHVPHLVPRVFYSDTEMAVTVMEDLSHLKIARKGLIEGENYPHLSQHIGEFLGKTLFYSSDYALEPKVKKQLVKQFTNPELCDITERLVFTDPFFDHDTNDFEEELRPFVEKLWNNDSVKIEAAKLKKSFLTSAE---TLIHGDLHTGSIFASEHE-TKVIDPEFAFYGPIGFDVGQFIANLFLNALSRDG---ADREPLYEHVNQVWETFEETFSEAWQKDSLDVYANIDGYLTDTLSHIFEEAIGFAGCELIRRTIGLAHVADLDTIVPFDKRIGRKRLALETGTAFIEKRSEFKTITDVIELFKLLVK

General information: TITO was launched using: RESULT: Template: 2OLC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1360 -41244 -30.33 -141.25 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -30.33 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.281

(partial model without unconserved sides chains): PDB file : Tito_2OLC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2olc-query.scw PDB file : Tito_Scwrl_2OLC.pdb: