Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKIYEKYRDLITNPFD-IKYKNIEIKEIISYPPAGNDVVECLCKIGKDARNVFIKIERSKV--CDFATEIKNLNYLKNNS-YYSKIPNIYESGIYNNKQYIVLSKIEGERLSDIL-IKNDN-LRKELLYKYGQELAIIHRIPISNQKIAKQRAINS--YPNKT---MYTTLSKE---NNIKEYIKFLEDNDFKKELTTFIHGDFHYANVLWLN-NDINGVIDWEYSGIGLREQDIAWACTLRPGQKFMDTMEDIQTFLKGYSEKEKFD--I--EKLRWCLINSYCHFY-LMNENNEDYKNKLLILLRNCMEEKI 5IQI Chain:C ((11-302)) ---NVKAMKYLIEHYFDNFKVD--SIEII-----GSGYDSVAYLVN----NEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLE---DSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIK--QEFIENGRKEIYKRT----

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1206 42070 34.88 154.67 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain C : 0.63 3D Compatibility (PKB) : 34.88 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.373

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: