Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMPQANIIPNDWWRWSDSQPV-IIKPLLGGLTNLNYLISVN---HELFVLRKNSAISEALNL-NRSAEAKALSRADEAGLCAPLIYYDDQHQYMVSRYLGD-KTWSVSIDHNLSLLAELLR-GIHQLPGIDADLNVENKISCYWQAIDAQAAFTRELISLDSAVGAHITSAKALNNGHV---LCHNDLLASNLIISNKDKLYAIDWEYAAMSDPFYELAVIIEG------NALNAKQQQSLLTRYLRQ----PISALDWQRLYHWQIIYGYLCVLWYAVQYSNGAMPNIVS--EIHRQILAIKALAAKDA---- 2PPQ Chain:A ((5-320)) -----TDITEDELRNFLTQYDVGSLTSYKGIAENSNFLLHTTKDPLILTLYEKKNDLPFFLGLMQHLAAKGLSCPLPLPRKDGELLGELSGRPAALISFLEGMWLRKPEAKHCREVGKALAAMHLASEGFEIKRPNALSVDGWKVLWDKSEERADEVEKGLREEIRPEIDYLAAHWPKDLPAGVIHADLFQDNVFFLGDELSGLIDFYFACNDLLAYDVSICLNAWCFEKDGAYNVTKGKALLEGYQSVRPLSEAELEALPLLSRGSALRFFLTRLYDWLTTPAGALVVKKDPLEYLRKLRFHRTIANVAEYGLA

General information: TITO was launched using: RESULT: Template: 2PPQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1308 -36312 -27.76 -127.86 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -27.76 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.287

(partial model without unconserved sides chains): PDB file : Tito_2PPQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ppq-query.scw PDB file : Tito_Scwrl_2PPQ.pdb: