Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVAAPIPVAGWGDKDDPWECLGERSSGATVYRVGE-VPSFYVKTTPPRHPDDHRFNPTKEAERLRWLAAQGLPVPEVVALDANDELAWVVTRALPGRPAARHWKPE-ERWRVIDVVADVARTLHALPVAECPFERRLADLIHQASSSMALGALDL--DDVDPSHEGWTAQQLWDELSKMTPPAEDDLVVCHGDFCLDNVLVDPETLTLAGILDVDRAGVSDRWMDLALALYNIGQDDVMGLRSAARRALPPAVRHHRRPAQADLHPTAGRISLS 3TM0 Chain:A ((20-259)) ------------------KDTEGM-SPAKVYKLVGENENLYLKMTDSRY-KGTTYDVEREKDMMLWLEG-KLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKT-EK-PEEELVFSHGDLGDSNIFVKDG--KVSGFIDLGRSGRADKWYDIAFCVRSIREDI---GEEQYVELFFDLLGIKPDWEKI------KYYILL

General information: TITO was launched using: RESULT: Template: 3TM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1067 34260 32.11 145.17 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 32.11 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.432

(partial model without unconserved sides chains): PDB file : Tito_3TM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tm0-query.scw PDB file : Tito_Scwrl_3TM0.pdb: