Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLTDQSTRIRAGEELDAAVIDPYLKAHIPGLEGTPKISQFPGGASNLTYLIEYP-RQEFVLRRPPFGHKAKSAHDMGREYRILNQLN-A-GFPYCPKAYLYCTDESVIGAEF----YVMERVKGIILRAELPPELNLDEQQTRSLCKSFIDKFVELHNVDYAACGLADLG--R---PDGYVQRQIAGWTDRYEKALTPDAPLWEPVKAWLKDKQPADHHKPGIVHNDYRFDNVILDPENPMQIIGVLDWELATLGDPLMDLGNTLAYWVEAGDPAPVQLTRRQPSHLPGMLTRREFADYYAERAGIPPIDNLDFYYTYGLFRLAGIVQQIYYRYYHGQTQDKRFAQFVQMNKLLEQMSLQAIERSRL 5IGH Chain:A ((6-259)) --------------TADTSQLYALAAR-HGLK-LHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAE---VSAKVEPEARVLAMLKNRLPFA-VPDWRVANAEL--VAYPMLEDSTAMVIQPGSSTPDWV------VPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFV----VNDKRLHRWQRWLDDDSSWPD-FSVVVHGDLYVGHVLIDNT--ERVSGMIDWSEARVDDPAIDMAAHLMVFGE--------------------EGLAKLLLTYEAAGGRVW-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 960 -56356 -58.70 -232.87 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -58.70 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.286

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: