Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTYTFDQVEKAIEQLYPDFTINTIEISGEGNDCIAYEINRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLP--LPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMED-DEEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSFEKIIYGKEYGYMDWYEEGLNEIRSIKIK 6CH4 Chain:D ((19-298)) ------------IEHYFDNFKVDSIEIIG-----VAYLVNNEYIFKTKF-------YAKEKAIYNFLNTNLETNVKIPNIEYSYISDE---LSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDI-SECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESF-MERLNATTVFEGKKCLCHNDFNCNHLLLDG-NNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEI------

General information: TITO was launched using: RESULT: Template: 6CH4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1181 25758 21.81 97.20 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain D : 0.77 3D Compatibility (PKB) : 21.81 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_6CH4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ch4-query.scw PDB file : Tito_Scwrl_6CH4.pdb: