Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVSQPVRSAAEWTAGDVAGVLAQACPAARVTSIRPGPPSYSNCLWLAETVDGRLLVRIPGRSADPEYVRATVTATHLASAA---GVPTVRYHEFVPQTPLGLPVTIQEYLPGESGTAA-L-RADRADLRLLASTLGDWLGTLHGVRRETFGAVTGSGERTTWTAAATDQVETALRAVPEAALPASPATIAAAFARVVAEL-GPGEPASLVHGDLYLDNMLVDN-GAPAALLDFEHAHYYDRFADFGKLSELLF-EWWPGAEEPFLESYRDHFPPDPADEVRLRLGVGLYALNQTAYFARWQPELVGEYRTRLGKWLAAG 5IQC Chain:A ((5-285)) ------YDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLV---NNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISD--ELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTID--NKQ--NVLEEYI-LLRETIY-NDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNI--DIEKAKEYQDIVEEYYPIETIVYGIKN-------------------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1217 -8725 -7.17 -31.96 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -7.17 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.349

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: