Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNIKESVQKLLGE-ELIVFELKGKGACNNAYYVETQSGKKFIIKEERPNGELKEQNSLAVEAHVIKQLSN-LNFSAPLAK-VYFIS--KEPNMFGYVYIEGDLLIDAW-KKLTEEERIGICQNLGVFHAELEQRVTEAMAREMGVQINT-YIGLHPEVEEEYSKILTFTD-IPN-GWKTLAQKTKTIFDK-TLELGVFQFLHNDAHHENIIVKD--GEITGIIDFGDSEYGEIAREFSRYIR---DF-PDHAEYIIKAYEKASGHKLSRERLISNSFLSGLMDNVEDYRKGGEDCIRAEKAVSKYEDIFT 3N4U Chain:A ((9-290)) ---VEKAIEQLYPDFTINTIEIS-GEGNDCIAYEIN--RDF-IFKFPKHSRG---STNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSN-L-V-LDFREKINEDNKKI---KKLLSRELKGPQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDI-PTVLEKY-RMKEKYWSFEKIIYGKEYGYMDWYEE-----------

General information: TITO was launched using: RESULT: Template: 3N4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1171 40797 34.84 153.37 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 34.84 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.329

(partial model without unconserved sides chains): PDB file : Tito_3N4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n4u-query.scw PDB file : Tito_Scwrl_3N4U.pdb: