Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLELDAKAISRIIQYLEPDLTISGFSRLEG-GSTEVYRIDLANSDYGSLVLKIYPDEP-EWGPSKEALVAGWLKD-LTLPVPTWLRVDESRSLLPLRYSLLTHLPGRSLRHWM----AEPDIRNVYRQMGELLRRIHAVPMPGYGYVRGHGIDKPLPTNADYMTAAFDDVFRRFRDLGGDPELAGRLQQFAEGSFDIFDASYGAVLAHDDLHQGNVLALRGK-AGGLELSGLVDFGNARAADKLFDLAKAIFCSAHEDPRSYQPILEGYGSVDHP-DTERALRLYTLFHRVSMWCWLTKIGVDVAAEDGPGGLIRDLSEMVV 3TDW Chain:A ((4-294)) -----NKLHYTTMIMTQFPDISIQSVESLGEGFRNYAILVN----G--DWVFRFPKSQQGADELNKEIQLLPLLVGCVKVNIPQYVYIGKR--SDGNPFVGYRKVQGQILGEDGMAVLPDDAKDRLALQLAEFMNELSAFPVETAISAGVP--VTNLKNKILLLSEAVEDQVFPLLDESLRDYLTLRFQSYMT--HPV-YTRYTPRLIHGDLSPDHFLTNLNSRQT--PLTGIIDFGDAAISDPDYDYVYLLEDC-GE---LFTRQVMAYRGEVDLDTHIRKVSLFVTFDQVSYLLEGLRARDQDWISEGLELLE--------

General information: TITO was launched using: RESULT: Template: 3TDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1259 -21440 -17.03 -76.03 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -17.03 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.349

(partial model without unconserved sides chains): PDB file : Tito_3TDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tdw-query.scw PDB file : Tito_Scwrl_3TDW.pdb: