Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --RCNVKIYNDKGKYQGQASDDW----GETLTIRGYTCYTDSYCKASC-DG----LPAGWTAEGT 1CSK Chain:A ((11-68)) GTEC-IAKYN----FHGTAEQDLPFCKGDVLTIVAVT-KDPNWYKAKNKVGREGIIPANYVQKR-

General information: TITO was launched using: RESULT: Template: 1CSK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 128 1611 12.59 34.28 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 12.59 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_1CSK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1csk-query.scw PDB file : Tito_Scwrl_1CSK.pdb: