Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTCYRGFLLGSCCRVAGGRAAALRGPGAGGPAARPRLGGDGGGRRHLGQGQPRELAGCGSRADGGFRPSRVVVVAKTTRYEFEQQRYRYAELSEEDLKQLLALKGSSYSGLLERHHIHTKNVEHIIDSLRNEGIEVRLVKRREYDEETVRWADAVIAAGGDGTMLLAASKVLDRLKPVIGVNTDPERSEGHLCLPVRYTHSFPEALQKFYRGEFRWLWRQRIRLYLEGTGINPVPVDLHEQQLSLNQHNRALNIERAHDERSEASGPQLLPVRALNEVFIGESLSSRASYYEISVDDGPWEKQKSSGLNLCTGTGSKAWSFNINRVATQAVEDVLNIAKRQGNLSLP-LNRELVEKVT--NEYNESLLYSPEEPKILFSIREPIANRVFSSSRQRCFSSKVCVRSRCWDACMVVDGGTSFEFNDGAIASMMINKEDELRTVLLEQ 1U0T Chain:B ((5-284)) --------------------------------------------------------------------RSVLLVVHTGRDEAT--E--T----ARRVEKVLGDNKIALRVLS-------------------------------ADQHAADGCELVLVLGGDGTFLRAAELARNASIPVLGVNL---GRIGFLAEA--EAEAIDAVLEHVVAQDYRVEDRLTLDVVVRQGG------------------------------------RIVNRGWALNEVSLEKGPRLGVLGVVVEIDGRPVSAFGCDGVLVSTPTGSTAYAFSAG-----------------GPVLWPDLEAILVVPNNAHALFGRPMVTSPEATIA------------------------IEIEADGHDALVFCDGRREMLIPAGSRLEVTRCVT-SVKWARL--

General information: TITO was launched using: RESULT: Template: 1U0T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1433 -96715 -67.49 -386.86 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -67.49 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.180

(partial model without unconserved sides chains): PDB file : Tito_1U0T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1u0t-query.scw PDB file : Tito_Scwrl_1U0T.pdb: