Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASVEVPQYVAEETTARWPNVGPQWVSEAPGELRQLCEQYGAIPETVLPARYGLVVAVQAKDR-ELIMKGSPDP--DGPNQVKVMSALADLQVGPTILESFSTD-------TGFWTIMTRIKPGEPLRNLGASLAPPDKLATILRPLVNQPSPSST--LPYIGDWLRDRLEDD-ALSDL---APG---RTVAS-------ETERSEALSVLS---------ELTDAG-AQGLCHGDTSPGNILTGENGKLYLIDPR-GMRGEAAYDVAVLGLKSAMT-----VSPETRVSDLAKAVGVDVGRAERWAAIALAARV 4PDY Chain:A ((18-293)) ---------------------------SQAPLIPQAVVSKYDLAIQQ-RH-ADGNIEVWTDSKGRRYAAKRSSIAPAHCRIMVQCLRHAQEQGFTKFARFVTTSSNAPYVRHGDFTYYVTEWVSGQPANF--GLPEHVAQTAYTLAQFHEATRSFRTDWK---DDVFGLFQARWRDLRQMWLGADRKREKDAFDQLLLSMRDELHRDAAESLALFEDRDVIAYLEAERSSGGWCHLDVIPSNCLYTPQHQVVLIDFELARPAPRALDMAHLLRRSLERGNWDGHLAYACFLHFDAVRNIPKSEYR-----------

General information: TITO was launched using: RESULT: Template: 4PDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 867 7466 8.61 32.60 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 8.61 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.300

(partial model without unconserved sides chains): PDB file : Tito_4PDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4pdy-query.scw PDB file : Tito_Scwrl_4PDY.pdb: