Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPSHLTHIWRLLKWGRTLARHGALTGIERDPLTPTPVRRLVRVARLGARVPRQPRYADAFQSIGPAAIKLGQTLATRPDL-VGEDAANDLLRLQDALPPVPFATIRAQMEQSFGRPLETLYSRFDEAPVGAASIAQVHRAVTTDGRDVAVKVIRPGVIDQFNRDIQTYEWAAAHVEMLGGEVARLRPRLVIANMKRWTARELDLRREAASASELGEAMEAMPGYRVPAIDWDRTTGKVMTMEWIDGVKISDRDALIAAGHDVKEIAARLVNAFLRQAIAEGFFHADMHQGNLFVTA-NGDIVAIDFGIMGRIDRRARMWLAEILYGLITGNYRRVAEIHFEAQYVPGHHNVEEFATA---LRAVGEP-MR-GKP--VRELSVGGMLDGLFAITRDFDMQTQPHLLL-LQKTMVMVEGVATALDPDINLWETSGPYVKEWLRAELGPEAKAADALIENVRILQRLPGLVKRIEEAFPEKGGAPPPPPLTEVRLIRVGSGWRYMLVALLAGLAGAGAMAAATTFL 4PED Chain:A ((4-389)) --------------------------------------------------ANAERIVRTLCKVRGAALKLGQMLSIQDDAFINPHLAKIFERVRQSADFMPLKQMMKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLARMKGGREVAMKIQYPGVAQSINSDVNNLMAVLNMSNML---PEGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHPFFYVPEIVDELCSPHVLTTELVSGFPLDQAEGL--SQEIRNEICYNILVLCLRELFEFHFMQTDPNWSNFFYDPQQHKVALLDFGATREYDRSFTDLYIQIIRAAADRDRETVRAKSIEMKFLTGY-EVKVMEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPVMLRHRLVPPPEETYSLHRKMGGSFLICSKLKARFPCKAMFEEAYSNYCKR---------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4PED.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2019 -16479 -8.16 -43.83 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -8.16 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_4PED.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ped-query.scw PDB file : Tito_Scwrl_4PED.pdb: