Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAERGRLLLTVVGELLAAEVCAAGTRIRGPDERARRERQRALRIRTALERLGPLYVKAGQVLSTREDL-VPPAVARELESLHDRAAPCPFDRMSAVLEAELGPDWRRRFRHFDTERPLGCASIAQAYAAVLDDGRSVVVKVQRPGIAAQMTTDMRLLRGMTRTLARRTPVLSETIDFEAMLAVIFEAVRNELDLTLEAANMDEARAAVTAVPGIDVPGVVHA--TERVLIQRRAPGTSVRDADPAAFDDAERERVGTGLLTVMYQGYFIDHRFHADPHPGNVFVCP-GGPTTLIDWGMIGRLDRHLSSTLLMTLLGLANNDAHAVARAWSEMGRPTPWADIGAFEQD---MSVVVPRLA--SI--PLDRLRFGASLATLLRLSTRRGIRTNPMIGL-LGKSFANMEGTVRHLAPHLSATDVLVRQTGRVLGEYAAQSLSQQQLGFTALQLLSGLETLLPHARAVGRSLSGGDLTLQHTAVTRPFSLVDHRSSARIRRAEHHLLGAAALVWLLRRRGRG 4PED Chain:A ((4-389)) ---------------------------------------ANAERIVRTLCKVRGAALKLGQMLSIQDDAFINPHLAKIFERVRQSADFMPLKQMMKTLNNDLGPNWRDKLEYFEE-RPFAAASIGQVHLARMKGGREVAMKIQYPGVAQSINSDVNNLMAVLNMSNML-P---EGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHPFFYVPEIVDELCSPHVLTTELVSGFPLDQAEGL--SQEIRNEICYNILVLCLRELFEFHFMQTDPNWSNFFYDPQQHKVALLDFGATREYDRSFTDLYIQIIRAAADRDRETVRAKSIEMKFLTGY-EVKVMEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPVMLRHRLVPPPEETYSLHRKMGGSFLICSKLKARFPCKAMFEEAYSNYCKR--------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4PED.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1994 -26730 -13.40 -71.47 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -13.40 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_4PED.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ped-query.scw PDB file : Tito_Scwrl_4PED.pdb: