Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPSPKLKLNLYQKLLGLDHAQFSLIQHEDAIVAIVYEVIEPSGKKLILKICEQE-----RHFLREVYFLEALKD--KIPVPQILQAIEPNSKRHGAILMEYLPGQVLNKETLTCELAEKAGYQLARLHLNSEKGYGDLIS--------KDSLTQDPKADLIQR-FEEGLDECE--AHLPSS---LLNECRHYFHTHLYLLELADGPCIIHRDFRPGNLLANKNKLLGIIDWASARAGFAQEDFSSLEMGEWSTQASIQAAFLSGYETIRPIPRY-QELMPLLKLSRSAAILGYLYRKGIYHKSSAKLHQSHLNFLKTFIEA 3F7W Chain:A ((2-287)) ---VNSVAARVTELTGREVA--AVAERGHSHRWHLYRVELADGTPLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQVAGWD------DRTLAMEWVDERPPT-----PEAAERFGHQLAAMHLAGAESFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAADRGALTPGDVRLVEKVLDALDHL---AGDPEPPARIHGDLWNGNVLWQDDG-AVVIDPA-AHGGHREADLAMLALFG----LPYLDRVRDAYNEVAPLAEGWRARIPLHQLHPLLVHVCLF---------GAAYRTTLVDTARAALR-

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1399 -28455 -20.34 -107.78 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -20.34 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: