Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLPRRPDADPAALLAGLDRSLAGLGLTEVGEGFDNIAYAVRSPSGARLLLRVSKVADPVERARVLTKDVALL----EFANRHSSLTTNEVIAADAHAGALLLTEVTGRTADQIRPTDRVAFAATMATFLTRLHTAPVVEAALVTEMDPRSAAQ----WLVDTATAWAAVADRTPVTLRDRVDEFLV--GPAPSDVRRITFCHNDLGDEHVVLSEDGGHVRGVIDWSDAVLGDPARDLGLLTLDFGPGIVDALIDDYHGPTGADFRGRVLWFAATAGVEGLAWRLANARPWQVTADRLHDVLAVLA 5UXD Chain:A ((18-248)) ----------------GLDLDGGTLRTEEIGLDF-RVAFA-RAHDGGDWVLRLPRRPDVLERAAVEGRLLAMLAPHLDVAVPDWRISTSELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAV-DAEAAAATGIEVRSPAQVRGAWRQDLARV-GAEFEIAP-ALRERWEAWLADDGCWPG---HSVLTHGELYPAHTLVEDE--RITAVLDWTTAAVGDPAKDLMFHQVSAPSAIFEVALQAY-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 861 -32467 -37.71 -146.91 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -37.71 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: