Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYPGINETQLELIFSRYG-KDCKIEPLQEEASTRRYFRITSVNGKEEVVCADET----VNEDFIVISEFLNANGIRVPRIHDTNQNLGLTFMSFEGLKDFSTYNLND---YKNKFPILIDLILKLQSLD-PPSLVKTRKFDTEKLSFETNL-TLDKFEAFRKQFQIKTDITNEA-KAFIEETVGYLNKYPINVFTHRDFHCRNILIAPN----TDYALIDFQDARMGVPQYDLASILYDAYYPLPRDFRSLMLKSFRERNVDQTKKFNDTFYLQALQRSFKALGTYFRMVTDHKKDKFKTSIISCLNQLEEIIQLGMFADSLYIFVRSLREELSRHKEFKNL 3CSV Chain:A ((6-322)) ------EDEIRDFLATHGYADWNRTP--------RYQRLRSPTGAKAVLMDWSPEEGGDTQPFVDLAQYLRNLDISAPEIYAEEHARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELA----RLDPETLSEMTRLAFSEYRYAI---LGDAAEDNRKRFEHRFAQILSAQLEGDM-VFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAHPA----LASAAEEILNALPA-------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1361 -9177 -6.74 -31.21 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -6.74 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: