Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHVPLSIHTTSYSMAAWDITTADE-----AWLIELCHKSADEGRQIG-GQEHGGPKVVRISDRIVAKY-GNIRRSELAAQEVAYHNTDRSIVHIPKVHRFFESDG----QSYLFMEYVEGQTLEDVD--FETHKDIPIRVANILAHLQQILGDCDSPGPVTGGEAHGYIFGDEGAGTAFDSIEDMNVYMNKRLDKMNEYLSRQEDQRRFDHLDLTPYALVLCHGDICRRNIIFESDGSLCLVDWGFAGFYPRIFELAAISYV-VQNNAAFKDPIIHEFT-KLLSLTDKEKQDIDRFRAVRFANLRWSFRDRRTTAEEDKFIQELYETQKRIKEEQETAGIQSFIISELLDGLLEQKSRPDLEVRHVPRPWTGSQTAIGHVKRVVVVGLDVNVA---SFILAMKILSSRRIFVIRTTQGSPK 1B6C Chain:B ((14-339)) ---------TTLKDLIYDMTTSGSGSGLPLLVQRTIARTIVLQESIGKGRFGEVWRGKWRGEEVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTVEGMIKLALSTASGLAHLHM---------------------------------------------------------EIVGTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVRHDSATDTIDIAPNHRVGTKRYMAPEVLDDSINMKHFESFKRADIYAMGLVF-----WEIARRCSIGGIHEDYQLPYYDLVP----------SDPSVEEMRKVVCEQKLRPNI-----PNRWQ-SCEALRVMAKIMRECWYANGAARLTALRIKKTLSQ-----LSQQEG---

General information: TITO was launched using: RESULT: Template: 1B6C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1645 29379 17.86 95.38 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : 17.86 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.174

(partial model without unconserved sides chains): PDB file : Tito_1B6C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1b6c-query.scw PDB file : Tito_Scwrl_1B6C.pdb: