Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDITLNYIKNNFAN-IINIVKPKNQGKLSCVYFADYNDTKIVIKIPMNA---GSLSKEIQFYELLKN-IELPSPKILSYNYKNIYRIPEYLIMTKLNGAPLHDNCC---NDSTKFKIYADIGIYLKMIHSINYFSYSTTFTPYSCDGVINFNSWIEYLNSEIGVILNKVQLSGIFSVYHVEKFRNHFKKLVNI--D-YN-LVLLHGDLGPDHLYFRH----DKFEGIIDPGLSFLGPKEYDLAYMAIYTDPHYFHKIASTYGPY-SQELVDSLMLLILSQKILRHFENEKVINIEKFINVISTYKLLD 3TDW Chain:A ((7-280)) --HYTTMIMTQFPDISIQSVESLGEGFRNYAILVN---GDWVFRFPKSQQGADELNKEIQLLPLLVGCVKVNIPQYVYIGKR--SDGNPFVGYRKVQGQILGEDGMAVLPDDAKDRLALQLAEFMNELSAFPVETAIS-A------GV-PVTNLKNKILLLSEAVEDQVF--PLLDESLRDYLTLRFQSYMTHPVYTRYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYLLEDCGE-LFTRQVMAYRGEVDLDTHIRKVSLFVTFDQVSYLLEGLRA----------------

General information: TITO was launched using: RESULT: Template: 3TDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1122 -35470 -31.61 -138.02 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -31.61 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_3TDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tdw-query.scw PDB file : Tito_Scwrl_3TDW.pdb: