Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGHPPPWTASPLFFLLLFWHAPPSLVSSCLHAGHALHVHPAHNPSLPPLSLPSDRPGAAGDGQTGASLVHNARAALPLCASRVFAGGALQPSTAVPPRTQPLHRCARRYAPSGRLLLKPRLGLKALPIHEVAEGRAEQEDRARPPDPSVSGRTLTCVTLRTKETCNDADCGGLACRTGNATIAGRGARASDGRQPPTKQEGVFPVATSFRFSSRSLRSSTPLSRYFLSSSPSENKFEFHHFLSYTVTAELPTSAFTFVDAPQSLTSCADVWSHPCRAFVFFPLQRSRTSVGFSPSSHSIRPSPRRWFSSVNLCALRASFGSIASSCSSPVGPDHSSLTAVAITRAVRCFPSHSAPFPPSSSCDLLSASSPSHASLPACSASASSSSPPSRARPTVCLYVPRTHSPRPPRSVGSAASPSSGGPRVSQSEGTPSGPVDSPLSPTSRGSCSYSTPSVSCPGGLLLSLPSVSPVSLSTSLRHFLVSYTSPFPARSLPLIFVDAGLSLASDASLHAARPVGLSQEHRETCASALGSLIDTAVASNAVAQTSCECRPRGLPIVYSPRLVPPSFPRNHCLQPQKLGRLFSFLTHPSGASNLRTPESGVTKTWEAREAEIAKATSGVEGSHEFRWDYVTVHSDLRPKRLNSDHPVTKAGIGVGENRGREKNGEDGRPSEAGCEKSTSPSHALSPSASVPHGPVDATRQNGCGRDDPPRCTHPAGVQVTAGNELNRNPLLAGSHDQHMDSTRATLGTSVSSCFSLFSPIHDADVTRAWLQVVHAPEYVCAASAAVLSE-E-----EERKIGFPVTKGYADKSLAEVSSTVLGTWLAFH----FGLACVVGGGTHHAKTDSGGKFCVFNDVAVAAALALKQ-GIAERILILDLDVHQGDGTAEIFSNEPRVKTVSIHCED---NFPFPK------------AQSDVDIGLPAG-TGDEVYLRQLNEVG 5WGI Chain:A ((26-255)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DSHHPELPQRISRIFSRHEE-------LRLLSRCHRIPARL---ATEEELALCHSSKHISIIKSSEHMKPRDLNRLGDEYNSIFISNESYTCALLAAGSCFNSAQAILTGQVRNAV-AIVRPPGHHAEKDTACGFCFFNTAALTARYAQSITRESLRVLIVDWDVHHGNGTQHIFEEDDSVLYISLHRYEDGAFFPNSEDANYDKVGLGKGRGYNVNIPWNGGKMGDPEYMAAFHHLV

General information: TITO was launched using: RESULT: Template: 5WGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 902 -25576 -28.35 -127.88 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -28.35 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.071

(partial model without unconserved sides chains): PDB file : Tito_5WGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5wgi-query.scw PDB file : Tito_Scwrl_5WGI.pdb: