Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLCKWTVLNSEG--EIIESGSCLNTEYMKHGDIHVEFE-NDCT-PIIFG----------- 3LZK Chain:A ((19-358)) FQGMKLATLKDSTRDGKLVVVSKDLTRCSEVGHIARTLQAALDDWAHAGPRLERVAEGIETGAQPTMRFHEHDAASPLPRAFQWADGSAYVNHVELVRKARNAEMPASFWTDPLIYQGGSDSFLGPRDPILMADDAWGIDMEGEAAVIVDDVPMGATLDEAKAAIRLVMLVNDVSLRGLIPGELAKGFGFYQSKPSSAFSPVAVTPEELGEAWDGGKLHLPLHVDLNGEPFGRANAGIDMTFDFPQLIVHAARTRPLSAGTIIGSGTVSNKLEGGPGRPVSEGGAGYSCIAELRMIETIEGGA-PKTQFLKFGD-VVRIEMKDRTGHSIFGAIEQKVGKYER

General information: TITO was launched using: RESULT: Template: 3LZK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 115 5149 44.77 119.73 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 44.77 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.019

(partial model without unconserved sides chains): PDB file : Tito_3LZK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3lzk-query.scw PDB file : Tito_Scwrl_3LZK.pdb: