Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------QIGKWVLSKEQIDALFFYQCQVTIHYPG-AERGKLLKYLAEPREPIPLPKFQGKLYYAISDSGCKYREYSKGDPWPKGL---------------------------------------------------------------------------------------------- 4F0D Chain:A ((2-271)) MGWAAAREAAGRDMLAADLRCSLFASALQSYKRDSVLRPFPASYARGDCKDFEALLADASKLPNLKELLQSSGDNHKRAWDLVSWILSSKV----------LTIHSAGKAEFEKIQKLTGAPHTPVPAPDFLFEIEYFDPANAKFYETKGERD-----LIYAFHGSRLENFHSIIHNGLHCHKTSLFGEGTYLTSDLSLALIYSPHGHGWQHSLLGPILSCVAVCEVIDHPDKYFVVTNNQLLRVKYLLVYSQ

General information: TITO was launched using: RESULT: Template: 4F0D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 111 2044 18.41 32.44 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 18.41 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_4F0D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4f0d-query.scw PDB file : Tito_Scwrl_4F0D.pdb: