Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -AQK--ICHFTILKMYKNTWTFVRKDSAPADQTTTIEGIKVFFSKDCIPQHDY 1NVP Chain:C ((30-76)) DTENVVVCQYDKIHRSKNKWKFHLKDG-----IMNLNGRDYIFSK-AIGDAEW

General information: TITO was launched using: RESULT: Template: 1NVP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 123 9490 77.15 215.68 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : 77.15 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.300

(partial model without unconserved sides chains): PDB file : Tito_1NVP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nvp-query.scw PDB file : Tito_Scwrl_1NVP.pdb: