Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGISNTNPPGGAPRPRQQCFLSADLGPDVMGQPTLLEATPFVNDMFDFHGYRCQMGKDCSHVRCRGFPAAFKMTAR------------------- 1B8G Chain:A ((1-428)) MLSRNATSSYFLGWQEYEKNPYHEVHNTNGIIQMGLAENQLCFDLLESWLAKNPEAAAFKKNGESIFAELALFQDYHGLPAFKKAMVDFMAEIRGNKVTFDPNHLVLTAGATSANETFIFCLADPGEAVLIPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITETALEEAYQEAEKRNLRVKGVLVTNPSNPLGTTMTRNELYLLLSFVEDKGIHLISDEIYSGTAFSSPSFISVMEVLKDRNCDENSEVWQRVHVVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSSFGLVSSQTQHLLSAMLSDKKLTKNYIAENHKRLKQRQKKLVSGLQKSGISCLNGNAGL-------FCWVDM-RHLLRSNTFEAEMELWKKIVYEVHLNISPGSSCH---CTE-PGWFRVCFANLPERTLDLAMQRLKAFVG

General information: TITO was launched using: RESULT: Template: 1B8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 58 -928 -16.00 -14.50 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -16.00 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.211

(partial model without unconserved sides chains): PDB file : Tito_1B8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1b8g-query.scw PDB file : Tito_Scwrl_1B8G.pdb: