Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------QDLRRPL-LCDVYIEYPSGQVFDQALGHDIVPAGETAEIS-GFKCKTVR-GTCV---- 1E2W Chain:A ((169-232)) TIYNASAAGKIVAITALSEKKGGFEVSIEKANGEVVV-----DKIPAGPDLIVKEG---QTVQADQPLTNNP

General information: TITO was launched using: RESULT: Template: 1E2W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 127 6963 54.83 161.93 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 54.83 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_1E2W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1e2w-query.scw PDB file : Tito_Scwrl_1E2W.pdb: