Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence QVPEATQMYAPGYDLNKAPCRIVIYKPTNPIGGTGEEYFMGNVAPGGVARFDGYICKASKDCLRPTCFNMPFGFRYFGRHM-- 2NCL Chain:A ((1-81)) ATQTEGELRVTQILKEKFP-RATAIKVTDISGGCGAMYEIKIESEEFKEKRTVQQHQMVNQALKEEIKEMH-GLRIFTSVPKR

General information: TITO was launched using: RESULT: Template: 2NCL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 338 -21140 -62.54 -267.59 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -62.54 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.074

(partial model without unconserved sides chains): PDB file : Tito_2NCL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ncl-query.scw PDB file : Tito_Scwrl_2NCL.pdb: