Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAVQMDPELAKRLFFEGATVVILNMPKGTEFGIDYNSWEVGPK--FRGVKMIPPG-IHFLHYSSVDKANPKEVGPRMGFFLSLHQRGLTVLRWSTLREEVDLSPAPESEVEAMRANLQELDQFLGPYPY-ATLKKWISLTNFISEATVEKLQPENRQICAFSDVLPVLSMKHTKDRVGQNLPRCGIECKSYQEGLARLPEMKPRAGTEIRFSELPTQMFPEGATPAEITKHSMDLSYALETVLNKQFPSSPQDVLGELQFAFVCFLLGNVYEAFEHWKRLLNLLCRSEAAMMKHHTLYINLISILYHQLGEIPADFFVDIVSQDNFLTSTLQVFFSSACSIAVDATLRKKAEKFQAHLTKKFRWDFAAEPEDCAPVVVELPEGIEMG 4ILG Chain:A ((1-337)) -----------------MNTVPFTSAPIEVTIGIDQYSFNVKENQPFHGIKDIPIGHVHVIHFQHADN-----SSMRYGYWFDCRMGN-FYIQYDPKDGLYKMMEERDGAKFENIVHNFKERQMMVSYPKIDEDDTWYNLTEFVQMDKIRKIVRKDENQFSYVDS----SM-TTVQ-------------------ENELSSSSSDPAHSLNYTVINFKS-REAIRPGHEMEDFLDKSYYLNTVMLQGIFKNSSNYFGELQFAFLNAMFFGNYGSSLQWHAMIELICSSAT-V--PKHMLDKLDEILYYQIKTLPEQY-SDILLNERVWNICLYSSFQKNS---LHNTEKIMENKYPELL-----------DDEHNPTIVGGL------

General information: TITO was launched using: RESULT: Template: 4ILG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1457 -67175 -46.10 -215.30 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -46.10 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.254

(partial model without unconserved sides chains): PDB file : Tito_4ILG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ilg-query.scw PDB file : Tito_Scwrl_4ILG.pdb: