Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRSRSRSSSRSKHTKSSKHNKKRSRSRSRSRDKERVRKRSKSRESKRNRRRESRSRSRSTNTAVSRRERDRERASSPPDRIDIFGRTVSKRSSLDEKQKREEEEKKAEFERQRKIRQQEIEEKLIEEETARRVEELVAKRVEEELEKRKDEIEREVLRRVEEAKRIMEKQLLEELERQRQAELAAQKAREEEERAKREELERILEENNRKIAEAQAKLAEEQLRIVEEQRKIHEERMKLEQERQRQQKEEQKIILGKGKSRPKLSFSLKTQD 5SZH Chain:A ((1-148)) --------------------------------------------------------------------------------------------------------GHMKQEELKRLYKAQAIQRQLEEVEERQRASEIQGVRLEKALRGEQD----EAQLLQEWFKLVLEKNKLMRYESELLIMAQELELEDHQSRLEQKLREKMLKEESQK----DEKDLNEEQEVFTELMQVIEQRDKLVDSLEEQRIREKAED------------------

General information: TITO was launched using: RESULT: Template: 5SZH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 225 21186 94.16 148.15 target 2D structure prediction score : 0.90 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 94.16 2D Compatibility (Sec. Struct. Predict.) : 0.90 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.299

(partial model without unconserved sides chains): PDB file : Tito_5SZH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5szh-query.scw PDB file : Tito_Scwrl_5SZH.pdb: