Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MEEFDSEDFSTSEEDEDYVPSGGEYSEDDVNELVKEDEVDGEEQTQKTQGKKRKAQSIPARKRRQGGLSLEEEEEEDANSESEGSSSEEEDDAAEQEKGIGSEDARKKKEDELWASF--------LNDVGPKSKVPPSTQVKKGEETEE--TSSSKLLVKAEELEKPKETE-------------------KVKITKVFDFAGEEVRVTKE-VDATSKEAKSFFKQNEKEKPQANVP--------SALPSLPAGSGLKRSSGMSS------------------------LLGKIGAKKQ-----KMSTLEKSKL------DWESFKE-----EEGIGEELAIHNRGKEGYIERKAF---LDRVDHRQFEIERDLRLSKMKP--- 5CUY Chain:A ((18-409)) DLASAEVAGLPVSALHGTNISTGRGSEADVAEPIQEAVDRKVSELDLAAYDKDDFT-QPMIKKIMSRLFSAFDVTHLG-YLTPDKVEEVCRYLGRNMSDGDVKAMKAEINAIDGHVTFEKFWAWWCSHPVHSRTKCFSMVSADFSMPYHQQQLVVHEKGEMYTPSYRVLYFFRDLETGRERQVSPWHDIPLYVRDLVRTKPEATPMNRYNFICEIPKWTRAKFEIATGESFNPIKQDIKNGVPRFYKHGDMMWNYGAFPQTWESTEVLFEAGVTGDNDPVDAVEIGMTQFKVGQVSAVKVLGVLGMIDEGEMDWKVVCISHSDPICHFLRDIHDVPKFLPGCLDAIREWFRVYKICQGGEASHFAFDGEFKDKEYAMKVIDESHNMWHNLRKVN

General information: TITO was launched using: RESULT: Template: 5CUY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1017 106176 104.40 357.49 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 104.40 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_5CUY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5cuy-query.scw PDB file : Tito_Scwrl_5CUY.pdb: