Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD 2FFX Chain:J ((1-173)) -SSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKH-

General information: TITO was launched using: RESULT: Template: 2FFX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 671 -62357 -92.93 -360.45 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain J : 0.99 3D Compatibility (PKB) : -92.93 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_2FFX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ffx-query.scw PDB file : Tito_Scwrl_2FFX.pdb: