@TOME V2.3
(Nov 2016)
Ref.
-
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Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : Lmo0137: (2016-03-12 )
MRLDFSKKTMQDFERDAEVVHATRERSFWRFMLADRRAVFATSVLILITVACIFAFLSPYDPNALSVQDKLMPPNSSHWFGTDDHGRDYLTRVLYGGRVSLLVGVFAMMIAVVVGTLAGTVSGYFGGFIDNIVMRFLDIFMSIPSFFLLMILNAYLKPGISNIIIIIGLLSWMEVARIVRAETLTLKEREFILYSKSSGGGFFHIMFKHIIPNAMPSIVVAASLNVATAILTESALSFLGLGVQQPNASWGSMLNNAQGYVGEATYLALFPGLLILMTILSFNVLGDVLRKGLSRRY
Atome Classification :
(20 SA)
--.....-....10........20.....----...30---......---..40.--.......------50........60...---------.....70........80........90-----........100.......110.......120....-------...130----........140.......150.....------.----------------.-------------------------160.......170.......180.......190.......200.......--210.......220.......230......-.240...---------....250----.......260.........270----.......280-----------........290......-------------------------------------------------------------------------------------------------------------------------------------------
(Atome)
(Ident)
(Tito)
(Num)
(Ligand)
(Uniprot)
--MRLDF-
S
K
K
T
M
Q
D
F
E
R
D
A
E
V
V
H
A
T
R
E
R
----
S
F
W
R
F
---
M
L
ADR
R
---
A
V
F
A
T
--
S
V
L
I
L
I
T
------
V
A
C
I
F
A
FLSPYDPNA---------LS
V
Q
DKLMPPNSSHWFGTDDH
G
R
D
Y
L
T
-----
R
V
L
YGG
R
V
S
L
L
V
G
V
F
A
M
M
I
A
V
V
V
G
T
L
A
G
T
V
S
G
Y
F
G
-------
G
F
I
D
N
-----
I
V
M
R
F
L
D
I
F
M
S
I
P
S
F
F
L
L
M
I
L
N
A
Y
LK------P----------------G-------------------------I
S
N
I
I
I
I
I
G
L
L
S
W
M
E
V
A
R
I
V
R
AE
T
L
T
L
KE
R
E
F
I
L
Y
SKSSGGG
F
F
H
I
M
F
K
H
--IIPNAM
P
S
I
V
V
A
AS
L
N
V
A
T
A
I
L
T
E
S
A
L
S
F
-
L
GLGVQQ---------PNA
S
W
G
S
----
M
LNNAQGY--VGE
A
T
Y
L
A
L
F
P
G
----
L
L
I
L
M
T
I
L
S
------------
F
N
V
L
G
D
V
L
R
K
-
G
LSRRY-------------------------------------------------------------------------------------------------------------------------------------------
20
Fugue
77.54
20%
-103
- C3 -
4US3
13
?
A:
[10-450]
--
G
R
E
Q
W
-
A
S
R
L
G
F
I
L
A
A
M
G
S
A
V
G
---
L
G
----
N
I
W
R
F
---
S
Y
V
T
G
E
N
GG
A
A
F
L
L
--
V
Y
L
G
F
I
A
LI
G
I
P
I
V
L
A
E
F
T
I
G
R
R
A
Q
S
D
A
---------
V
G
S
F
E
K
L
A
P
G
K
P
W
K
V
A
G
L
M
G
V
A
A
G
F
L
I
-----
L
S
F
Y
G
V
I
A
G
W
I
L
F
Y
L
F
N
Y
I
T
---
G
Q
L
W
S
A
P
A
E
G
F
G
-------
G
F
F
E
G
-----
F
I
A
N
P
-----
T
L
P
L
F
W
Q
A
L
F
M
I
A
T
I
-----------------------------------------------------
W
I
V
A
I
G
V
K
K
G
I
E
R
S
N
K
I
L
M
P
L
L
G
V
L
L
I
A
L
A
I
Y
S
L
T
L
G
G
--
A
K
E
G
L
A
F
--
L
F
S
---
P
D
W
S
A
L
K
D
P
G
V
Y
L
A
A
I
S
Q
A
F
F
T
L
S
L
G
M
G
A
L
I
---------
T
Y
G
S
Y
V
S
KDSR
L
P
G
A
A
V
S
V
--
A
G
L
D
T
A
F
A
I
I
A
G
----
I
M
I
F
P
A
V
F
A
L
GLSPSGGPG
L
V
F
V
V
L
P
D
I
F
D
S
I
R
L
G
P
I
V
G
I
A
F
F
I
L
L
G
A
A
A
L
S
S
A
V
S
L
L
E
V
P
V
A
Y
F
M
R
K
F
DWS
R
K
Q
A
A
I
T
L
G
V
I
I
T
L
L
G
I
P
S
S
L
S
F
GVLGEVTIIPGLN
I
F
D
S
V
D
F
I
A
SSV
F
L
P
L
G
G
M
I
I
A
L
F
I
G
WGWK
T
S
D
A
L
A
E
S
DLTDS
V
W
G
K
L
W
I
L
S
L
R
F
I
A
P
I
A
I
L
I
V
F
L
S
A
F
10
HHSearch
76.78
18%
-129
- C3 -
3TUI
-
METI_ECOLI
A:
[2-216]
-------------------------------------------------------------------------------------------------------------------
S
E
P
M
M
W
-----
L
L
V
R
G
V
W
E
T
L
A
M
T
F
V
S
G
F
F
G
F
V
I
G
L
P
V
G
V
L
L
Y
V
T
R
PGQIIAN
A
K
L
Y
R
-----
T
V
S
A
I
V
N
I
F
R
S
I
P
F
I
I
L
L
V
W
M
I
P
F
T
R
V
I
VGTS
I
----------------
G
-------------------------
L
Q
A
A
I
V
P
L
T
V
G
A
A
P
F
I
A
R
M
V
E
-
N
A
L
L
E
I
P
T
G
L
I
E
A
S
R
A
M
G
A
T
P
M
Q
I
V
R
K
V
--
L
L
P
E
A
L
P
G
L
V
N
A
A
T
I
T
L
I
T
L
V
G
Y
S
A
M
G
G
A
-
V
G
---------------
A
G
G
L
G
Q
----
I
G
Y
Q
Y
G
Y
I
--
G
Y
-
N
A
T
V
M
N
T
V
L
----
V
L
L
V
I
L
V
Y
L
------------
I
Q
F
A
G
D
R
I
V
R
-
A
V
T
R
---------------------------------------------------------------------------------------------------------------------------------------------
21
Fugue
76.28
17%
-134
- C3 -
4YMU
2
?
C:
[1-214]
--
M
T
V
D
F
------------------------------
L
---
S
M
V
K
Y
T
---
P
L
F
I
S
--
G
L
I
M
T
L
K
------
L
T
F
L
A
V
T
I
G
V
L
M
G
L
F
---------
I
A
L
M
K
M
-------------------------------
S
S
I
K
P
I
K
L
V
A
S
S
Y
I
E
V
I
R
G
T
---------------------
P
-----
L
L
V
Q
L
L
L
I
Y
N
G
L
M
Q
F
G
M
N
I
-------------
P
----------------
A
-------------------------
F
T
A
G
V
S
A
L
A
I
N
S
S
A
Y
V
A
E
I
I
R
A
G
I
Q
A
V
-
D
P
G
Q
N
E
A
A
R
S
L
G
M
T
H
A
M
A
M
R
Y
V
--
I
I
P
Q
A
I
K
N
I
L
P
A
L
G
N
E
F
I
V
M
L
K
E
S
A
I
V
S
V
-
I
G
F
A
D
-----------------------
L
T
R
Q
A
D
I
--
I
Q
S
V
T
Y
R
Y
F
E
P
Y
----
I
I
I
A
A
I
Y
F
V
------------
M
T
L
T
F
S
K
L
L
S
-
L
F
E
R
R
L
-------------------------------------------------------------------------------------------------------------------------------------------
8
HHSearch
71.52
17%
-133
- C3 -
3D31
-
?
C:
[78-283]
------------------------------------------------------------------------------------------------------------------------
R
-----
S
V
I
S
S
I
F
L
S
L
Y
A
G
F
L
A
T
L
L
A
L
L
L
G
A
P
T
G
Y
I
L
A
R
F
D
-------
F
P
G
K
R
-----
L
V
E
S
I
I
D
V
P
V
V
V
P
H
T
V
A
G
I
A
L
L
T
V
F
G
------
S
RGLIGEPLESYIQFRD
A
-------------------------
L
P
G
I
V
V
A
M
L
F
V
S
M
P
Y
L
A
N
S
A
R
-
E
G
F
K
S
V
D
P
R
L
E
N
A
A
R
S
L
G
A
P
L
W
K
A
F
F
F
V
--
T
L
P
L
S
A
R
Y
L
L
I
G
S
V
M
T
W
A
R
A
I
S
E
F
G
A
V
V
I
-
L
A
Y
Y
-------------
P
M
V
G
P
T
----
L
I
Y
D
R
F
I
S
--
Y
G
--
L
S
A
S
R
P
I
A
----
V
L
L
I
L
V
T
L
S
------------
I
F
L
V
I
R
------------------------------------------------------------------------------------------------------------------------------------------------------
9
HHSearch
71.26
14%
-128
- C3 -
2ONK
-
WTPB_ARCFU
C:
[68-275]
------------------------------------------------------------------------------------------------------------------------
P
-----
A
V
W
K
V
V
L
T
T
Y
Y
A
A
L
I
S
T
L
I
A
V
I
F
G
T
P
L
A
Y
I
L
A
R
K
S
-------
F
P
G
K
S
-----
V
V
E
G
I
V
D
L
P
V
V
I
P
H
T
V
A
G
I
A
L
L
V
V
F
G
------
S
----------------
S
GLIGSFSPLKFVDA-----------
L
P
G
I
V
V
A
M
L
F
V
S
V
P
I
Y
I
N
Q
A
K
-
E
G
F
A
S
V
D
V
R
L
E
H
V
A
R
T
L
G
S
S
P
L
R
V
F
F
T
V
--
S
L
P
L
S
V
R
H
I
V
A
G
A
I
M
S
W
A
R
G
I
S
E
F
G
A
V
V
V
-
I
A
Y
Y
-------------
P
M
I
A
P
T
----
L
I
Y
E
R
Y
L
S
--
E
G
--
L
S
A
A
M
P
V
A
----
A
I
L
I
L
L
S
L
A
------------
V
F
V
A
L
R
I
I
V
G
--------------------------------------------------------------------------------------------------------------------------------------------------
3
PsiBlast_PDB
70.30
24%
-155
- C3 -
3TUZ
-
METI_ECOLI
A:
[19-158]
-------------------------------------------------------------------------------------------------------------------------------------
M
T
F
V
S
G
F
F
G
F
V
I
G
L
P
V
G
V
L
L
Y
V
T
R
P
--
G
-------
Q
I
I
A
N
A
K
L
Y
R
T
V
S
A
I
V
N
I
F
R
S
I
P
F
I
I
L
L
V
W
M
I
P
F
T
R
------
V
I
VGTSI----------
G
-------------------------
L
Q
A
A
I
V
P
L
T
V
G
A
A
P
F
I
A
R
M
V
E
N
A
L
L
E
I
P
T
-
G
L
I
E
A
S
R
A
M
G
A
T
P
M
Q
I
V
R
K
V
--
L
L
P
E
A
L
P
G
L
V
N
A
A
T
I
T
L
I
T
L
V
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
2
PsiBlast_PDB
69.63
24%
-155
- C3 -
3TUI
-
METI_ECOLI
A:
[19-158]
-------------------------------------------------------------------------------------------------------------------------------------
M
T
F
V
S
G
F
F
G
F
V
I
G
L
P
V
G
V
L
L
Y
V
T
R
P
--
G
-------
Q
I
I
A
N
A
K
L
Y
R
T
V
S
A
I
V
N
I
F
R
S
I
P
F
I
I
L
L
V
W
M
I
P
F
T
R
------
V
I
VGTSI----------
G
-------------------------
L
Q
A
A
I
V
P
L
T
V
G
A
A
P
F
I
A
R
M
V
E
N
A
L
L
E
I
P
T
-
G
L
I
E
A
S
R
A
M
G
A
T
P
M
Q
I
V
R
K
V
--
L
L
P
E
A
L
P
G
L
V
N
A
A
T
I
T
L
I
T
L
V
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
16
Fugue
68.12
13%
-120
- C3 -
2D0B
1
?
A:
[2-309]
S
N
Y
V
I
Q
A
-
D
Q
Q
L
L
D
A
L
R
A
H
Y
E
G
A
L
S
D
R
L
P
----
A
G
A
L
F
---
A
V
K
R
P
D
---
V
V
I
T
A
Y
R
S
G
K
V
L
F
Q
------
G
K
A
A
E
Q
E
A
A
K
W
I
S
G
A
S
A
S
NETADH
Q
P
S
A
L
A
A
H
Q
L
G
S
L
S
A
I
G
S
D
E
V
G
T
G
---------
D
Y
F
G
P
I
V
V
A
A
A
Y
V
D
R
P
H
I
A
K
I
A
A
L
G
V
K
D
S
K
Q
L
N
D
-------
E
A
I
K
R
-----
I
A
P
A
I
M
E
T
V
P
H
A
V
T
V
L
D
N
P
Q
Y
N
R
W
Q
R
------
S
----------------
G
-------------------------
M
P
--
Q
T
K
M
K
A
L
L
H
N
R
T
L
V
K
L
V
D
A
I
A
P
A
E
P
E
A
I
I
I
D
E
F
L
K
R
D
S
Y
F
R
Y
L
S
D
E
DR
I
I
R
E
R
V
H
C
L
P
K
A
E
S
V
H
V
S
V
A
A
A
S
I
I
A
R
Y
V
-
F
L
E
E
M
E
Q
---------
L
S
R
A
V
G
L
----
L
L
P
K
G
A
G
A
--
I
V
D
E
A
A
A
R
I
I
R
A
RG
E
E
M
L
E
T
C
A
K
L
H
------------
F
A
N
T
K
K
A
L
A
I
-
A
K
R
R
---------------------------------------------------------------------------------------------------------------------------------------------
24
Fugue
67.42
15%
-93
- C3 -
2ONK
-
WTPB_ARCFU
C:
[24-275]
--
M
R
L
L
F
-
S
A
L
L
A
L
L
S
S
I
I
L
L
F
V
L
L
P
V
A
A
----
T
V
T
L
Q
LFN
F
D
E
F
L
K
---
A
A
S
D
P
--
A
V
W
K
V
V
L
------
T
T
Y
Y
A
A
L
I
S
T
L
I
A
V
I
---------
F
G
T
P
L
A
Y
I
L
A
R
K
S
-----------
F
P
G
-----
K
S
V
V
E
G
I
V
D
L
P
V
V
I
P
H
T
V
A
G
I
A
L
L
V
V
F
G
S
-
S
G
L
I
G
-------
S
F
S
P
-------------------------
L
K
F
V
D
A
L
------
P
----------------
G
-------------------------
I
V
V
A
M
L
F
V
S
V
P
I
Y
I
N
Q
A
K
E
G
F
A
S
V
----
D
V
R
L
E
H
V
A
R
T
L
G
S
S
P
L
R
V
F
F
T
V
--
S
L
P
L
S
V
R
H
I
V
A
G
A
I
M
S
W
A
R
G
I
S
E
F
G
A
V
V
V
-
I
A
-
Y
Y
P
M
---------
I
A
P
T
L
I
Y
----
E
R
Y
L
S
E
G
L
--
S
A
A
M
P
V
A
A
I
L
I
L
----
L
S
L
A
V
F
V
A
L
------------
R
I
I
V
G
-------------------------------------------------------------------------------------------------------------------------------------------------------
1
PsiBlast_PDB
66.29
24%
-153
- C3 -
3DHW
-
METI_ECOLI
A:
[19-158]
-------------------------------------------------------------------------------------------------------------------------------------
M
T
F
V
S
G
F
F
G
F
V
I
G
L
P
V
G
V
L
L
Y
V
T
R
P
--
G
-------
Q
I
I
A
N
AKLY
R
T
V
S
A
I
V
N
I
F
R
S
I
P
F
I
I
L
L
V
W
M
I
P
F
T
R
------
V
I
VGTSI----------
G
-------------------------
L
Q
A
A
I
V
P
L
T
V
G
A
A
P
F
I
A
R
M
V
E
N
A
L
L
E
I
P
T
-
G
L
I
E
A
S
R
A
M
G
A
T
P
M
Q
I
V
R
K
V
--
L
L
P
E
A
L
P
G
L
V
N
A
A
T
I
T
L
I
T
L
V
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
7
PsiBlast_PDB
64.76
24%
-109
- C3 -
3TUJ
-
METI_ECOLI
A:
[20-158]
--------------------------------------------------------------------------------------------------------------------------------------
T
F
V
S
G
F
F
G
F
V
I
G
L
P
V
G
V
L
L
Y
V
T
R
P
--
G
-------
Q
I
I
A
N
A
K
L
Y
R
T
V
S
A
I
V
N
I
F
R
S
I
P
F
I
I
L
L
V
W
M
I
P
F
T
R
------
V
I
V
GTSI----------
G
-------------------------
L
Q
A
A
I
V
P
L
T
V
G
A
A
P
F
I
A
R
M
V
E
N
A
L
L
E
I
P
T
-
G
L
I
E
A
S
R
A
M
G
A
T
P
M
Q
I
V
R
K
V
--
L
L
P
E
A
L
P
G
L
V
N
A
A
T
I
T
L
I
T
L
V
-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
11
HHSearch
63.39
14%
-122
- C3 -
3RLF
2
MALF_ECOLI
F:
[252-503]
------------------------------------------------------------------------------------------------------
S
P
G
Y
T
V
T
T
G
W
K
N
F
T
R
V
F
T
D
EGIQK
P
F
L
A
I
F
V
W
T
V
V
F
S
L
I
T
V
F
L
T
V
A
V
G
M
V
L
A
C
L
V
Q
W
E
A
-------
L
R
G
K
A
-----
V
Y
R
V
L
L
I
L
P
Y
A
V
P
S
F
I
S
I
L
I
F
K
G
L
F
N
------
Q
----------------
S
FGEINMMLSALFGVKPAWFSDPTTA
R
T
M
L
I
I
V
N
T
W
L
G
Y
P
Y
M
M
I
L
C
M
-
G
L
L
K
A
I
P
D
D
L
Y
E
A
S
A
M
D
G
A
G
P
F
Q
N
F
F
K
I
--
T
L
P
L
L
I
K
P
L
T
P
L
M
I
A
S
F
A
F
N
F
N
N
F
V
L
I
Q
L
-
L
T
N
G
G
P
D
RLGTTTPAG
Y
T
D
L
L
V
N
----
Y
T
Y
R
I
A
F
E
GG
G
G
Q
D
F
G
L
A
A
A
I
A
----
T
L
I
F
L
L
V
G
A
------------
L
A
I
---------------------------------------------------------------------------------------------------------------------------------------------------------
25
Fugue
51.52
19%
-34
- C3 -
2C9L
2
BZLF1_EBVB9
Y:
[1-63]
------------
M
L
E
I
K
R
Y
K
N
R
V
A
A
R
K
S
R
----
A
K
F
K
Q
---
L
L
Q
H
Y
R
---
E
V
A
A
A
--
K
S
S
E
N
D
R
------
L
R
L
L
L
K
Q
M
C
P
----
S
---------
L
D
V
D
S
I
I
P
R
T
P
D
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
19
Fugue
43.68
22%
187
- C3 -
2L58
-
HRK_HUMAN
A:
[1-32]
---
R
L
G
L
R
S
S
A
A
Q
L
T
A
A
R
L
K
A
L
G
D
E
L
H
Q
R
----
T
M
W
R
R
---
R
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
22
Fugue
42.79
23%
166
* C3 *
3EFD
-
?
K:
[129-158]
------
S
-
E
K
A
A
E
E
A
Y
T
R
T
T
R
A
L
H
E
R
F
D
R
------
L
E
R
---
M
L
D
D
N
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
23
Fugue
39.09
9%
105
- C3 -
2Y0M
-
B:
[29-70]
---
D
I
L
E
-
N
L
D
D
S
V
F
S
K
R
H
A
K
L
E
L
D
E
K
R
R
----
K
R
W
D
I
---
Q
R
I
R
E
Q
---
R
I
L
Q
R
--
L
---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
4
PsiBlast_PDB
38.33
27%
-22
- C3 -
1FQY
-
AQP1_HUMAN
A:
[8-50]
----------------------------
K
----
L
F
W
R
A
---
V
V
A
E
------
F
L
A
T
--
T
L
F
V
F
I
S
------
I
G
S
A
L
G
F
K
Y
P
V
G
N
N
Q
---------
T
A
V
Q
D
N
V
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
18
Fugue
38.16
21%
285
- C3 -
1BY0
-
SHDAG_HDVAM
A:
[1-27]
---------
R
K
K
L
E
E
L
E
R
D
L
R
K
L
K
K
K
I
K
K
LEED
N
P
W
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
6
PsiBlast_PDB
35.78
27%
-50
- C3 -
1H6I
-
AQP1_HUMAN
A:
[9-50]
---------------------------------
L
F
W
R
A
---
V
V
A
E
------
F
L
A
T
--
T
L
F
V
F
I
S
------
I
G
S
A
L
G
F
K
Y
P
V
G
N
N
Q
---------
T
A
V
Q
D
N
V
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
17
Fugue
34.07
19%
182
- C3 -
2M5E
-
B:
[1-26]
---------------
K
R
K
Q
E
E
V
S
A
I
V
I
Q
R
----
A
Y
R
R
Y
---
L
L
K
Q
K
V
---
K
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------